Position: Professor

School/Academic Group: School of Pharmaceutical Sciences

Email: yu_yi@whu.edu.cn

Education：

2003.09 - 2007.06  Institute of Microbiology, Chinese Academy of Sciences，Ph.D.

Professional Memberships and/or Notable Appointments:

Traditional Chinese Pharmacy Programs, Ministry of Education, Member of the Teaching Advisory Committee

Botanical Society of China, Medicinal Plant & Phytomedicine Section，Member of Council

Traditional Chinese Medicine and Natural Medicinal Products Committee, Hubei Pharmaceutical Association, Member of the Standing Committee

Chinese Traditional and Herbal Drugs, Member of the Youth Editorial Board

Frontiers in Microbiology, Associate Editor

Working Experience：

2003.09 - 2007.06  Shanghai Institute of Organic Science, Chinese Academy of Sciences，Collaborative researcher

2007.07 - 2009.06  Discipline of Bioengineering, School of Life Sciences and  Biotechnology, Shanghai Jiao Tong University，Lecturer

2009.07 - 2010.06  College of Life Science and Technology, Huazhong Agricultural University，Lecturer

2020.10 - now   Wuhan University, Professor (During this period, Visiting  Scholar at Washington University in St. Louis in 2013 and University of Oxford in 2018)

Current Teaching Interests/Responsibilities:

Immunology; Chemical biology

Research Interests:

Chemical biology and synthetic biology of bioactive natural products in medicinal plants

Publications：

1. Linrui Wu^#, Qian Zhang^#, Zixin Deng, Yi Yu*, From solo to duet, intersections of natural product assembly with self-resistance, Natural Product Reports, 2022, DOI: 10.1039/d1np00064

2. Shan Wang^#, William D G Brittain^#, Qian Zhang^#, Zhou Lu, Ming Him Tong, Kewen Wu, Kwaku Kyeremeh, Matthew Jenner, Yi Yu*, Steven L Cobb*, Hai Deng*, Aminoacyl chain translocation catalysed by a type II thioesterase domain in an unusual non-ribosomal peptide synthetase, Nature Communications, 2022, 13(1):62

3. Qian Zhang^#, Hao-Tian Chi^#, Linrui Wu, Zixin Deng, Yi Yu*. Two Cryptic Self-Resistance Mechanisms in Streptomyces tenebrarius Reveal Insights into the Biosynthesis of Apramycin. Angew. Chem. Int. Ed. Engl., 2021, 60(16):8990-8996

4. Yating Liu^#, Linrui Wu^#, Zixin Deng, Yi Yu*. Two putative parallel pathways for naringenin biosynthesis in Epimedium wushanense. RSC Advances, 2021, 11, 13919 – 13927

5. Fleurdeliz Maglangit*, Yi Yu*, Hai Deng*. Bacterial pathogens: threat or treat (a review on bioactive natural products from bacterial pathogens). Natural Product Reports, 2021, 38(4):782-821

6. Haigang Song, Cong Rao, Zixin Deng, Yi Yu*, James Naismith*. The biosynthetic pathway of the benzoxazole in nataxazole proceeds via an unstable ester and has synthetic utility. Angew. Chem. Int. Ed. 2020, 59, 2–11

7. Lie Ma^#, Siyao Sun^#, Ziyu Yuan, Zixin Deng, Yi Yu*. Three putative DNA replication/repair elements encoding genes confer self-resistance to distamycin in Streptomyces netropsis. Acta Biochimica et Biophysica Sinica. 2019, 10.1093/abbs/gmz133

8. Yating Liu^#, Li Su^#, Qing Fang, Jioji Tabudravu, Xiaohui Yang, Kirstie Rickaby, Laurent Trembleau, Kwaku Kyeremeh, Zixin Deng, Hai Deng*, Yi Yu*. Enzymatic reconstitution and biosynthetic investigation of the bacterial carbazole neocarazostatin A. Journal of Organic Chemistry, 2019, 84, 24, 16323-16328

9. Yingying Chang, Baozhong Chai, Yunkun Ding, Min He, Linghui Zheng, Yun Teng, Zixin Deng, Yi Yu*, Tiangang Liu*. Overproduction of gentamicin B in industrial strain Micromonospora echinospora CCTCC M 2018898 by cloning of the missing genes genR and genS. Metabolic Engineering Communications, 2019, 9, e00096

10. Yun X, Zhang Q, Lv M, Deng H, Deng Z, Yu Y.* In vitro reconstitution of the biosynthetic pathway of 3-hydroxypicolinic acid. Organic & Biomolecular Chemistry, 2019, 17(3):454-460

11. Ding W., Ji W., Wu Y., Wu R., Liu W., Ma X., Zhang W., Xu P., Deng Z., Tang B., Yu Y.*, Zhang Q*. Biosynthesis of the nosiheptide indole side ring centers on a cryptic carrier protein NosJ. Nature Communications, 2017, 8(1):437. DOI: 10.1038/s41467-017-00439-1

12. Yu Y., Zhang Q., Deng Z., Parallel pathways in the biosynthesis of aminoglycoside antibiotics. F1000Res. 2017, doi: 10.12688/ f1000research.11104.1

13. Su Li, Zhang R, Kyeremeh K, Deng Z, Deng H* and Yu Y*. Dissection of the neocarazostatin A C4 alkyl side chain biosynthesis by in vitro reconstitution. Organic & Biomolecular Chemistry, 2017, 15(18): 3843-3848

14. Ding W, Li Y, Zhao J, Ji X, Mo T, Qianzhu H, Tu T, Deng Z, Yu Y, Chen F, Zhang Q*. The Catalytic Mechanism of the Class C Radical S-Adenosylmethionine Methyltransferase NosN. Angew Chem Int Ed Engl., 2017, 56(14):3857-3861

15. Ding W, Wu Y, Ji X, Qianzhu H, Chen F, Deng Z, Yu Y, Zhang Q*. Nucleoside-linked shunt products in the reaction catalyzed by the class C radical S-adenosylmethionine methyltransferase NosN. Chem Commun (Camb)., 2017, 53(37):5235-5238

16. Su Li, Lv Meinan, Kyeremeh Kwaku, Deng Zixin, Deng Hai and Yu Yi *. A ThDP-dependent enzymatic carboligation reaction involved in Neocarazostatin A tricyclic carbazole formation.Organic & Biomolecular Chemistry, 2016, 14(37): 8679-8684

17. Lv M, Ji X, Zhao J, Li Y, Zhang C, Su L, Ding W, Deng Z, Yu Yi* and Zhang Q*. Characterization of a C3 Deoxygenation Pathway Reveals a Key Branch Point in Aminoglycoside Biosynthesis. J AM CHEM SOC, 2016, 138(20):6427-35

18. Huang S., Elsayed S., Lv M., Tabudravu J., Rateb M., Gyampoh R., Kyeremyh K., Ebel R., Jaspars M., Deng Z., Yu Yi* and Deng H.* Biosynthesis of neocarazostatin A reveals the sequential carbazole prenylation and hydroxylation in the tailoring steps. Chemistry & Biology, 2015, 22(12):1633-42

19. Lv Meinan, Zhao Junfeng, Deng Zixin and Yu Yi*. Characterization of the biosynthetic gene cluster for benzoxazole antibiotics A33853 reveals unusual assembly logic. Chemistry & Biology, 2015, 22(10):1313-1324.

20. Huang S, Tabudravu J, Elsayed SS, Travert J, Peace D, Tong MH, Kyeremeh K, Kelly SM, Trembleau L, Ebel R, Jaspars M, Yu Yi* and Deng H*. Discovery of a Single Monooxygenase that Catalyzes Carbamate Formation and Ring Contraction in the Biosynthesis of the Legonmycins. Angew Chem Int Ed Engl. 2015, 54(43):12697-12701

21. Huang S, Tong MH, Qin Z, Deng Z, Deng H, Yu Y.*. Identification and characterization of the biosynthetic gene cluster of thiolutin, a tumor angiogenesis inhibitor, in Saccharothrix algeriensisNRRL B-24137. Anticancer Agents Med Chem, 2015, 15(3):277-84.

22. Liu G, Zhang M, Mo T, He L, Zhang W, Yu Y*, Zhang Q*, Ding W*. Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus. Biochem Biophys Res Commun., 2015, 467(4):1058-62

23. Long Ma, Axel Bartholome, Ming Him Tong, Zhiwei Qin, Yi Yu, Thomas Shepherd, Kwaku Kyeremeh, Hai Deng* and David O'Hagan*. Identification of a fluorometabolite from Streptomyces sp. MA37: (2R3S4S)-5-fluoro-2,3,4-tri-hydroxypentanoic acid. Chemical Science, 2015, 6(2):1414-1419

24. Hao C.L., Huang S., Deng Z.X., Zhao C.M., Yu Y.* Mining of the pyrrolamide antibiotics analogs in Streptomyces netropsis reveals the amidohydrolase-dependent "iterative strategy" underlying the pyrrole polymerization. PLOS ONE, 2014, 9(6):e99077.

25. Qin Zhiwei, Wang Xiaoling, Rateb Mostafa, Ass'ad Lina, Jaspars Marcel, Deng Zixin, Yu Yi, Deng Hai*. Disruption of a Methyltransferase Gene in Actinomycin G Gene Cluster in Streptomyces iakyrus Increases the Production of Phenazinomycin. FEMS Microbiology Letters, 2014, 352(1): 62-8

26. Sheng Huang, Long Ma, Ming Him Tong, Yi Yu*, David O'Hagan and Hai Deng* Fluoroacetate biosynthesis from the marine-derived bacterium Streptomyces xinghaiensis NRRL B-24674. Org Biomol Chem, 2014, 12(27): 4828-4831

27. Varman, A.M. ^#, Yu, Y. ^#, You L., Tang, Y.J. Photoautotrophic production of D-lactate in an engineered cyanobacterium. Microbial Cell Factories, 2013, 12: 117

28. Qin, Z., Huang, S., Yu, Y.*, Deng, H*. Dithiolopyrrolone natural products: isolation, synthesis and biosynthesis. Marine Drugs, 2013, 11(10):3970-97

29. Yu, Y., You, L., Liu, D.Y., Hollinshead, W., Tang, Y.J., Zhang, F.Z. Development of Synechocystissp. PCC 6803 as a Phototrophic Cell Factory. Marine Drugs, 2013, 11(8):2894-916

30. Wang, X., Tabudravu, J., Rateb, M.E., Annand, K.J., Qin, Z., Jaspars, M., Deng, Z., Yu, Y.*, Deng, H*. Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus. Molecular Biosystems, 2013, 9(6):1286-9

31. Wu XM, Yu Y., Han LB, Li CL, Wang HY, Zhong NQ, Yao Y, Xia GX*.The tobacco BLADE-ON-PETIOLE2 gene mediates differentiation of the corolla abscission zone by controlling longitudinal cell expansion. Plant Physiology, 2012, 159(2):835-50

32. Zhang, Q.; Yu, Y.*, Thioether Crosslinkages Created by a Radical SAM Enzyme. ChemBioChem, 2012, 13(8), 1097-1099

33. Huang, S.; Zhao, Y.D.; Qin, Z.W.; Wang, X.L.; Onega, M.; Chen, L.; He, J.; Yu, Y.*; Deng, H.*, Identification and heterologous expression of the biosynthetic gene cluster for holomycin produced by Streptomyces clavuligerus. Process Biochemistry, 2011, 46, 811-16

34. Zhang, Q.; Li, Y.X.; Chen, D.D.; Yu, Y.; Duan, L.; Shen, B.; Liu, W. Radical-mediated enzymatic carbon chain fragmentation-recombination. Nature Chemical Biology, 2011, 7, 154-60

35. Yu, Y.; Guo, H.; Zhang, Q.; Duan, L.; Ding, Y.; Liao, R.J.; Lei, C.; Shen, B.; Liu, W. NosA Catalyzing Carboxyl-Terminal Amide Formation in Nosiheptide Maturation via an Enamine Dealkylation on the Serine-Extended Precursor Peptide. J AM CHEM SOC, 2010, 132(46), 16324-26.

36. Wang, J.; Yu, Y.; Tang, K.; Liu, W.; He, X.; Huang, X.; Deng, Z. Thiopeptide antibiotic cyclothiazomycin: Identification and Analysis of its biosynthetic gene cluster in Streptomyces hygroscopicus 10-22. (2010) Appl Environ Microb, 76(7), 2335-44.

37. Ding, Y.; Yu, Y.; Pan, H.; Guo, H.; Li, Y.; Liu, W. Moving posttranslational modifications forward to biosynthesize the glycosylated thiopeptide nocathiacin I in Nocardia sp. ATCC202099. (2010) Molecular Biosystems, 6(7), 1180-85.

38. Yu, Y.; Duan, L.; Zhang, Q.; Liao, R. J.; Ding, Y.; Pan, H. X.; Wendt-Pienkowski, E.; Tang, G. L.; Shen, B.; Liu, W., Nosiheptide Biosynthesis Featuring a Unique Indole Side Ring Formation on the Characteristic Thiopeptide Framework. (2009) ACS Chem Biol, 4(10), 855-64.

39. Liao, R. J.; Duan, L.; Lei, C.; Pan, H. X.; Ding, Y.; Zhang, Q.; Chen, D. J.; Shen, B.; Yu, Y.*; Liu, W.* Thiopeptide Biosynthesis Featuring Ribosomally Synthesized Precursor Peptides and Conserved Posttranslational Modifications. (2009) Chemisty & Biology, 16 (2), 141-47.

40. Liu, W.; Yu, Y. Combinatorial Biosynthesis of Pharmaceutical Natural Products. Biocatalysis for the Pharmaceutical Industry: Discovery, Development, and Manufacturing (Chapter 11) Wiley Press, ISBN: 978-0-470-82314-9, Oct. 2008, 229-246

41. Yu, Y.; Wang, H.Y.; Liu, L.N.; Chen, Z.L.; Xia, G.X. Functional identification of cytokinesis-related genes from tobacco BY-2 cells. (2007) Plant Cell Rep, 26(7), 889-94

42. Chen, Z.L.^#; Yu, Y.^#; Liu, L.N.; Xia, G.X. Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells. (2007) Progress in Nature Science 17, 156-62. (^#Contributed equally)

43. Yu, Y.; Bai, L. Q.; Minagawa, K.; Jian, X. H.; Li, L.; Li, J. L.; Chen, S. Y.; Cao, E. H.; Mahmud, T.; Floss, H. G.; Zhou, X. F.; Deng, Z. X., Gene cluster responsible for validamycin biosynthesis in Streptomyces hygroscopicus subsp jinggangensis 5008. (2005) Appl Environ Microb, 71 (9), 5066-76.

44. Wang, H.Y. ^#; Yu, Y. ^#, Chen, Z.L.; Xia, G.X. Functional characterization of Gossypium hirsutum profilin 1 gene (GhPFN1) in tobacco suspension cells. Characterization of in vivo functions of a cotton profilin gene. (2005) Planta 222, 594-603. (^#Contributed equally)

45. 王岩；虞沂；赵群飞；孔毅；刘文，天然产物的生物合成和组合生物合成研究进展。国外医药（抗生素分册）2008，29（6），275-282

46. 张华；赵群飞；虞沂；陆大年；刘文，特异性克隆编码多肽合成酶还原结构域基因的PCR方法。中国抗生素杂志 2009，34（3），189-192

47. 吕美男；虞沂^*，2-脱氧链霉胺类抗生素的生物合成研究进展。中国科技论文在线, 2014

48. 张玉；虞沂^*，吡咯酰胺类化合物合成途径中醛基转移酶Pya26的功能研究。太阳成集团tyc234cc学报（理学版），2016，62（1）：078-084

49. 黄胜；虞沂^*，二硫吡咯酮类抗生素的生物合成研究与代谢工程应用。微生物学报（微生物药物创新专刊），2016，3