Position: Professor

School/Academic Group: School of Pharmaceutical Sciences

Email: weili.mn@whu.edu.cn

Education：

1996.09 - 2000.07  Huazhong Agricultural University，Bachelor

1999.09 - 2002.07  Huazhong Agricultural University，Master

2004.08 - 2010.08  Iowa State University，Ph.D.

Working Experience：

2002.09 – 2004.08  Shanghai Genomics, Inc. Research Assistant

2010.08 - 2012.11  Iowa State University，Postdoc

2012.12 - 2017.03 Purdue University，Postdoc

2017.04 - 2018.10 Purdue University，Assistant Research Scientist

2018.11 - 2020.11  Wuhan University，Special Reseracher

2020.12 - now     Wuhan University，Professor

Research Interests:

The structure and function of biomacromolecules

Publications：

1. Huang, W., Y. He, R. Jiang, Z. Deng, F. Long*. (2022) Functional and Structural Dissection of a Plant Steroid 3-O-Glycosyltransferase Facilitated Engineering Enhancement of Sugar Donor Promiscuity. ACS Catalysis. 12(5):2927-2937

2. Sevvana, M., T. F. Rogers, A. S. Miller, F. Long, T. Klose, N. Beutler, Y. C. Lai, M. Parren, L. M. Walker, G. Buda, D. R. Burton, M. G. Rossmann, R. J. Kuhn. (2020) Structural basis of zika virus specific neutralization in subsequent flavivirus infections. Viruses. 12(12):1346

3. Long, F., M. Doyle, E. Fernandez, A. S. Miller, T. Klose, M. Sevvana, A. Bryan, E. Davidson, B. J. Doranz, R. J. Kuhn, M. S. Diamond, J. E. Crowe, M. G. Rossmann. (2019) Structural basis of a potent human monoclonal antibody against Zika virus targeting a quaternary epitope. Proc. Natl. Acad. Sci. USA.116 (5):1591-1596

4. Sevvana, M., F. Long, A. Miller, T. Klose, G. Buda, L. Sun, R. J. Kuhn, and M. G. Rossmann. (2018) Refinement and analysis of the mature zika virus Cryo-EM structure at 3.1 resolution. Structure. 26(9):1169-1177.e3

5. Hasan, S. S., A. Miller, G. Sapparapu, T. Klose, F. Long, E. Fernandez, J. C. Porta, W. Jiang, M. S. Diamond, J. E. Crowe, Jr., R. J. Kuhn, M. G. Rossmann. (2016) A potently neutralizing human antibody cross-links Zika virus to prevent infection. Nat. Commun. 8:14722

6. Long, F., R. H. Fong, S. K. Austin, Z. Chen, T. Klose, A. Fokine, Y. Liu, J. Porta, G. Sapparapu, W. Akahata, B. J. Doranz, J. E. Crowe, M. S. Diamond, M. G. Rossmann. (2015) Cryo-EM structures elucidate neutralizing mechanisms of anti-chikungunya human monoclonal antibodies with therapeutic activity. Proc. Natl. Acad. Sci. USA 112(45):13898-13903

7. Fox, J. M., F. Long, M. A. Edeling, H. Lin, M. Duijl-Richter, R. H. Fong, K. M. Kahle, J. M. Smith, J. Jin, G. Simmons, B. J. Doranz, J. E. Crowe, D. H. Fremont, M. G. Rossmann, M. S. Diamond. (2015) Broadly neutralizing alphavirus antibodies bind an epitope on E2 and inhibit entry and egress. Cell163(5):1095-1107

8. Su, C.-C.#, Long, F.#, M. T. Zimmermann, R. L. Jernigan, and E. W. Yu. (2011) Crystal structure of the CusBA heavy-metal efflux complex of Escherichia coli. Nature 470:558-562.

9. Long, F.#, Su, C.-C.#, M. T. Zimmermann, S. E. Boyken, K. R. Rajashankar, R. L. Jernigan, and E. W. Yu. (2010) Crystal structures of the CusA efflux pump suggest methionine-mediated metal transport. Nature 467:484-488.

10. Liu, Y., J. Sheng, A. Fokine, G. Meng, W. H. Shin, F. Long, R. J. Kuhn, D. Kihara, M. G. Rossmann. (2015) Structure and inhibition of EV-D68, a virus that causes respiratory illness in children. Science347(6217):71-74.

11. Do, S. V., C.-C. Su, F. Long, H.-T. Lei, J. R. Bolla and E. W. Yu. (2013) Structure, mechanism and assembly of the tripartite CusCBA heavy metal efflux complex. Microbial Efflux Pumps: Current Research :51.

12. Long, F., C.-C. Su, H.-T. Lei, J. Bolla, S. V. Do, and E. W. Yu. (2012) Structure and mechanism of the tripartite CusCBA heavy-metal efflux complex. Philosophical Transactions B 367:1047-1058.  

13. Su, C.-C. #, Long, F. #, H.-T. Lei, J. Bolla, S. V. Do, K. R. Rajashankar and E. W. Yu. (2012) Charged amino acids (R83, E567, D617, E625, R669 and K678) of CusA are required for metal-ion transport in the Cus efflux system. J. Mol. Biol. 422:429-441.

14. Bolla, J. R., S. V. Do, F. Long, L. Dai, C.-C. Su, H.-T. Lei, X. Chen, J. E. Gerkey, D. C. Murphy, K. R. Rajashankar, and E. W. Yu. (2012) Structural and functional analysis of the transcriptional regulator Rv3066 of Mycobacterium tuberculosis. Nucl. Acids Res. 40(18):9340-9355

15. Bolla, J. R., Su, C.-C., J. A. Delmar, A. Radhakrishnan, N. Kumar, T.H. Chou, F. Long, K. R. Rajashankar, E. W. Yu. (2015) Crystal structure of the Alcanivorax borkumensis YdaH transporter reveals an unusual topology. Nat. Commun. 6:6874

16. Su, C.-C., Radhakrishnan, A., Kumar, N., F. Long, J. R. Bolla, H.-T. Lei, J. A. Delmar, S. V. Do, T.-H. Chou, K. R. Rajashankar, Q Zhang, and E. W. Yu. (2014) Crystal structure of the Campylobacter jejuni CmeC outer membrane channel. Prot. Sci. 23: 954–961.

17. Long, F., C. Rouquette-Loughlin, W. M. Shafer, and E. W. Yu. (2008) Functional cloning and characterization of the multidrug efflux pumps NorM from Neisseria gonorrhoeae and YdhE from Escherichia coli. Antimicrob. Agents Chemother. 52(9):3052-60.

18. Su, C.-C., Bolla, J. R., N. Kumar, A. Radhakrishnan, F. Long, J. A. Delmar, T.-H. Chou, K. R. Rajashankar, W. M. Shafer, E. W. Yu. (2015) Structure and function of Neisseria gonorrhoeae MtrF illuminates a class of antimetabolite efflux pumps. Cell Rep. 11(1):61-70

19. Lei, H-T, Chou, T-H, Su, C-C, J. R. Bolla, N. Kumar, A. Radhakrishnan, F. Long, J. A. Delmar, S. V. Do, K. R. Rajashankar, W. M. Shafer, E. W. Yu. (2014) Crystal structure of the open state of the Neisseria gonorrhoeae MtrE outer membrane channel. PLoS ONE 9(6): e97475.

20. Bolla, J. R., Su, C.-C., Do, S. V., A. Radhakrishnan, N. Kumar, F. Long, T.-H. Chou, J. A. Delmar, H.-T. Lei, K. R. Rajashankar, W. M. Shafer, E. W. Yu. (2014) Crystal structure of the Neisseria gonorrhoeae MtrD inner membrane multidrug efflux pump. PLoS ONE 9(6): e97903.

21. Su, C.-C., F. Long, and E. W. Yu. (2012) CusBA heavy metal efflux pump of E. coli. Encyclopedia of Inorganic and Bioinorganic Chemistry DOI: 10.1002/9781119951438.eibc2054.

22. Routh, M. D., Y. Zalucki, C.-C. Su, F. Long, Q. Zhang, W. M. Shafer, and E. W. Yu. (2011) Efflux pumps of the resistance-nodulation-division family: a perspective of their structure, function, and regulation in Gram-negative bacteria. Advances in Enzymology and Related Areas of Molecular Biology 77:109-146.

23. Su, C.-C., F. Long, and E. W. Yu. (2010) The Cus system removes toxic metal ions via a methionine shuttle. Prot. Sci. 20:6-18.

24. Su, C.-C., F. Yang, F. Long, D. Reyon, M. D. Routh, D. W. Kuo, A. K. Mokhtari, J. D. Van Ornam, K. L. Rabe, J. A. Hoy, Y. J. Lee, K. R. Rajashankar, and E. W. Yu. (2009) Crystal structure of the membrane fusion protein CusB from Escherichia coli. J. Mol. Biol. 393(2):342-55.

25. Su, C.-C., F. Long, G. McDermott, W. M. Shafer, and E. W. Yu. (2008) Crystallization and preliminary X-ray diffraction analysis of the multidrug efflux transporter NorM from Neisseria gonorrhoeae. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64:289-92.

26. Gu, R., M. Li, C.-C. Su, F. Long, M .D. Routh, F. Yang, G. McDermott, and E. W. Yu. (2008) Conformational change of the AcrR regulator reveals a possible mechanism of induction. Acta Crystallogr Sect. F Struct. Biol. Cryst. Commun. 64:584-8.

27. Li, M., X. Qiu, C.-C. Su, F. Long, R. Gu, G. McDermott, and E. W. Yu. (2006) Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the regulator AcrR from Escherichia coli. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62:1150-2.

28. Takano, E., Tao, M., F. Long, M. J. Bibb, L. Wang, W. Li, M. J. Buttner, M. J. Bibb, Z. X. Deng, and K. F. Chater. (2003) A rare leucine codon in adpA is implicated in the morphological defect of bldA mutants of Streptomyces coelicolor. Mol. Microbiol. 50(2):475-86.)