Position: Professor, Doctoral supervisor
School/Academic Group: School of Pharmaceutical Sciences
Email: gaolei0408@whu.edu.cn
Education：
2009.09 - 2013.06 Central China Normal University，Bachelor
2017.01 - 2017.11 Hokkaido University，Visiting student
2013.09 - 2019.01 National Institute of Biological Sciences, Beijing/Peking Union Medical College，Ph.D.
Working Experience：
2019.04 - 2021.09 College of Chemistry, Peking University，Postdoc
2021.10 - 2024.07 College of Chemistry, Peking University，Special Associate Researcher
2024.08 - now Wuhan University, Professor
Research Interests:
Complete Pathway Elucidation and Heterologous Reconstitution for Bioactive Natural Products in Plants;
Mining, Characterization, Mechanistic Elucidation, and Functional Engineering of Novel Enzymatic Catalytic Elements;
Chemoenzymatic Synthesis of Bioactive Natural Products and Drug Molecules
Publications：
1. Ding, Q.#, Guo, N.#, Gao, L.*, McKee, M., Wu, D., Yang, J., Fan, J., Weng, J.-K., Lei, X.* The evolutionary origin of naturally occurring intermolecular Diels-Alderases from Morus alba. Nat. Commun. 2024, 15, 2492.
2. Zhang, H., Xie, S., Yang, J., Ye, N., Gao, F., Gallou, F., Gao, L.*, Lei, X.* Chemoenzymatic synthesis of 2-aryl thiazolines from 4-hydroxybenzaldehydes using vanillyl alcohol oxidases. Angew. Chem. Int. Ed. 2024, 63, e202405833.
3. Jiang, B.#, Gao, L.#, Wang, H. #, Sun, Y. #, Zhang, X., Ke, H., Liu, S., Ma, P., Liao, Q, Wang, Y., Wang, H., Liu, Y., Du, R., Rogge, T., Li, W., Shang, Y., Houk, K. N., Xiong, X., Xie, D., Huang, S., Lei, X. *, Yan, J. * Characterization and heterologous reconstitution of Taxus biosynthetic enzymes leading to baccatin III. Science 2024, 383, 622-629.
4. Gao, L., Ding, Q. Lei, X.* Hunting for the intermolecualr Diels-Alderase. Acc. Chem. Res., 2024, 10.1021/acs.accounts.4c00315.
5. Wang, J. Ke, H., Yang, J. Guo, N., Hu, K., Tang, R., Ding, Q., Gao, L.*, Lei, X.* Diversity-oriented synthesis of cyclohexenes by combining enzymatic intermolecular Diels-Alder reactions and decarboxylative functionalizations. Chem. Catal. 2023, 3, 100451.
6. Gao, L.#, Zou, Y. #, Liu, X. #, Yang, J. #, Du, X., Wang, J., Yu, X., Fan, J., Jiang, M., Li, Y., Houk, K. N. *, Lei, X. * Enzymatic control of endo and exo stereoselective Diels-Alder reactions with broad substrate scope. Nat. Catal. 2021, 4, 1059-1069.
7. Gao, L.#, Su, C.#, Du, X.#, Wang, R.#, Chen, S., Zhou, Y., Liu, C., Liu, X., Tian, R., Zhang, L., Xie, K., Chen, S., Guo, Q., Guo, L., Hano, Y., Shimazaki, M., Minami, A., Oikawa, H., Huang, N., Houk, K. N., Huang, L.*, Dai, J.*, Lei, X*. FAD-dependent enzyme-catalysed intermolecular [4+2] cycloaddition in natural product biosynthesis. Nat. Chem. 2020, 12, 620-628.
8. Guo, Q.#, Wu, D.#, Gao, L.#, Bai, Y., Liu, Y., Guo, N., Du., X., Yang, J., Wang, X. Lei, X.* Identification of the AMA synthase from the Aspergillomarasmine A biosynthesis and evaluation of its biocatalytic potential. ACS. Catal. 2020, 10, 6291-6298.
9. Gao, L., Yang, J., Lei, X.* Enzymatic intermolecular Diels-Alder reactions in synthesis: From nature to design. Tetrahedron Chem 2022, 2,100013.
10. Gao, L*. Lei. X*. Biosynthetic intermediate probes for visualizing and identifying the biosynthetic enzymes of plant metabolites. ChembioChem, 2021, 22, 982.
11. Gao, L., Han, J. Lei, X.* Enantioselective total syntheses of kuwanons X and Y, kuwanol A. Org. Lett., 2016, 18, 360-363.
12. Liu, X. #, Yang, J.#. Gao, L.#, Zhang, L. Lei, X*. Chemoenzyamtic total syntheses of artonin I with an intermolecular Diels-Alderase. Biotechnol. J. 2020, 15, 2000119.
13. Gao, L.; Narita, K.; Ozaki, Kumakura, N.; Gan, P.; Minami, A.; Liu, C.; Lei, X.; Shirasu, K.; Oikawa, H.*“Identification of novel sesterterpenes by genome mining of phytopathogenic fungi Phoma and Colletotrichum sp”. Tetrahedron Lett. 2018, 59, 1136-1139.
